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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
20/08/2018 |
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Data da última atualização: |
22/08/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NUNES, G. F. de O.; MENEZES, K. A. S.; SAMPAIO, A. A.; LEITE, J.; FERNANDES JUNIOR, P. I.; SEIDO, S. L.; ZILLI, J. E.; MARTINS, L. M. V. |
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Afiliação: |
GERSIKA FAKIRRA DE OLIVEIRA NUNES, UNEB; KELLY ALEXSANDRA SOUZA MENEZES, UFAL; ALINE ARAÚJO SAMPAIO, UFBA; JAKSON LEITE, UFAL; PAULO IVAN FERNANDES JUNIOR, CPATSA; SIRANDO LIMA SEIDO, UFRPE; JERRI EDSON ZILLI, CNPAB; LINDETE MÍRIA VIEIRA MARTINS, UNEB. |
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Título: |
Polyphasic characterization of forage legumes root nodule bacteria isolated from semiarid region in Brazil. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Revista de Ciências Agrárias, Lisboa, v. 41, n. 3, p. 612-624, jul./set. 2018. |
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DOI: |
http://dx.doi.org/10.19084/RCA17339 |
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Idioma: |
Português |
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Conteúdo: |
Forage legumes are important resources in semiarid regions due to their abilities to adapt to soils with low fertility and fix nitrogen when associated with diazotrophic bacteria. Here, we applied a polyphasic approach to characterize a set of legume nodule isolates obtained from Clitoria ternatea and Stylosllntlzes capitata cultivated in the soils of a semiarid region of Brazil. A tolal of 126 bacterial isolates were obtained: 45 isolates frorn C. ternatea and 81 isolates from S. capitata. Nodulation tests revealed only ten isolates that nodulated their original host: six isolates from C. iernaiea and four isolates from S. capitata. These ten legume nodule isolates were phenotypically and genotypically characterized. All isolates grew in fructose, glucose, sodium glutamate, maltose, xylose. and sucrose. Metabolic tests showed a relationship between tolerance to salt and high temperaturas, where isolates that tolerated the highest salt concentration also tolerated the highest ternperature. Three isolates showed amylolytic activity, and four isolates showed carboxymethyl cellulolytic activity. 5treptomycin was the most limiting and nalidixic acid was the least limiting antibiotic to bacterial growth. Seven out of ten isolates were indol-acetic acid producers. Additionally, 165 rRNA gene partial sequencing enabled the identification of members of the genera Bacillus (1), Bradvrhizobium (4), Leifeonia (3), Microvirga (1), and Rhizobium (1). These data reveal phenotypically and genotypically di verse bacteria inhabiting the nodules of the forage legumes C. tematea and S. capitata represent an important microbial source to protect new biotechnological products and improve forage legumes in semiarid regions. MenosForage legumes are important resources in semiarid regions due to their abilities to adapt to soils with low fertility and fix nitrogen when associated with diazotrophic bacteria. Here, we applied a polyphasic approach to characterize a set of legume nodule isolates obtained from Clitoria ternatea and Stylosllntlzes capitata cultivated in the soils of a semiarid region of Brazil. A tolal of 126 bacterial isolates were obtained: 45 isolates frorn C. ternatea and 81 isolates from S. capitata. Nodulation tests revealed only ten isolates that nodulated their original host: six isolates from C. iernaiea and four isolates from S. capitata. These ten legume nodule isolates were phenotypically and genotypically characterized. All isolates grew in fructose, glucose, sodium glutamate, maltose, xylose. and sucrose. Metabolic tests showed a relationship between tolerance to salt and high temperaturas, where isolates that tolerated the highest salt concentration also tolerated the highest ternperature. Three isolates showed amylolytic activity, and four isolates showed carboxymethyl cellulolytic activity. 5treptomycin was the most limiting and nalidixic acid was the least limiting antibiotic to bacterial growth. Seven out of ten isolates were indol-acetic acid producers. Additionally, 165 rRNA gene partial sequencing enabled the identification of members of the genera Bacillus (1), Bradvrhizobium (4), Leifeonia (3), Microvirga (1), and Rhizobium (1). These data reveal phenotypically and ... Mostrar Tudo |
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Palavras-Chave: |
Fixação biológica do nitrogênio. |
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Thesagro: |
Clitoria Ternatea; Controle Biológico; Fixação de Nitrogênio; Leguminosa Forrageira. |
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Thesaurus Nal: |
Biological control; Bradyrhizobium; Forage legumes; Nitrogen fixation; Stylosanthes capitata. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/181656/1/RCA-vol41-n3-art04-RCA17339.pdf
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Marc: |
LEADER 02820naa a2200337 a 4500
001 2094257
005 2018-08-22
008 2018 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.19084/RCA17339$2DOI
100 1 $aNUNES, G. F. de O.
245 $aPolyphasic characterization of forage legumes root nodule bacteria isolated from semiarid region in Brazil.$h[electronic resource]
260 $c2018
520 $aForage legumes are important resources in semiarid regions due to their abilities to adapt to soils with low fertility and fix nitrogen when associated with diazotrophic bacteria. Here, we applied a polyphasic approach to characterize a set of legume nodule isolates obtained from Clitoria ternatea and Stylosllntlzes capitata cultivated in the soils of a semiarid region of Brazil. A tolal of 126 bacterial isolates were obtained: 45 isolates frorn C. ternatea and 81 isolates from S. capitata. Nodulation tests revealed only ten isolates that nodulated their original host: six isolates from C. iernaiea and four isolates from S. capitata. These ten legume nodule isolates were phenotypically and genotypically characterized. All isolates grew in fructose, glucose, sodium glutamate, maltose, xylose. and sucrose. Metabolic tests showed a relationship between tolerance to salt and high temperaturas, where isolates that tolerated the highest salt concentration also tolerated the highest ternperature. Three isolates showed amylolytic activity, and four isolates showed carboxymethyl cellulolytic activity. 5treptomycin was the most limiting and nalidixic acid was the least limiting antibiotic to bacterial growth. Seven out of ten isolates were indol-acetic acid producers. Additionally, 165 rRNA gene partial sequencing enabled the identification of members of the genera Bacillus (1), Bradvrhizobium (4), Leifeonia (3), Microvirga (1), and Rhizobium (1). These data reveal phenotypically and genotypically di verse bacteria inhabiting the nodules of the forage legumes C. tematea and S. capitata represent an important microbial source to protect new biotechnological products and improve forage legumes in semiarid regions.
650 $aBiological control
650 $aBradyrhizobium
650 $aForage legumes
650 $aNitrogen fixation
650 $aStylosanthes capitata
650 $aClitoria Ternatea
650 $aControle Biológico
650 $aFixação de Nitrogênio
650 $aLeguminosa Forrageira
653 $aFixação biológica do nitrogênio
700 1 $aMENEZES, K. A. S.
700 1 $aSAMPAIO, A. A.
700 1 $aLEITE, J.
700 1 $aFERNANDES JUNIOR, P. I.
700 1 $aSEIDO, S. L.
700 1 $aZILLI, J. E.
700 1 $aMARTINS, L. M. V.
773 $tRevista de Ciências Agrárias, Lisboa$gv. 41, n. 3, p. 612-624, jul./set. 2018.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sul. Para informações adicionais entre em contato com cppsul.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sul. |
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Data corrente: |
06/12/2022 |
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Data da última atualização: |
06/12/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MARÍN GÓMEZ, A.; LACA, E. A.; BALDISSERA, T. C.; PINTO, C. E.; GARAGORRY, F. C.; ZUBIETA, A. S.; BREMM, C.; BINDELLE, J.; CARVALHO, P. C. de F. |
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Afiliação: |
ALEJANDRA MARÍN GÓMEZ, Federal University of Rio Grande do Sul; EMILIO A. LACA, University of California; TIAGO CELSO BALDISSERA, EPAGRI; CASSIANO EDUARDO PINTO, EPAGRI; FABIO CERVO GARAGORRY, CPPSUL; ANGEL S. ZUBIETA, Federal University of Rio Grande do Sul; CAROLINA BREMM, Federal University of Rio Grande do Sul; JERÔME BINDELLE, Liège University; PAULO CÉSAR DE FACCIO CARVALHO, Federal University of Rio Grande do Sul. |
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Título: |
Determining the pre-grazing sward height of Kikuyu grass (Cenchrus clandestinus - Hochst. ex Chiov.) for optimizing nutrient intake rate of dairy heifers. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
PLos One, v. 17, n. 7, e0269716, July 2022. |
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DOI: |
https://doi. org/10.1371/journal.pone.0269716 |
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Idioma: |
Inglês |
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Conteúdo: |
Understanding the grazing process and animal response to sward structures (e.g., sward height) is key to setting targets for efficient grazing management. We hypothesized that the short-term intake rate (STIR) of dry matter (DM) and digestible organic matter (OM) by dairy heifers is maximized with Kikuyu grass (Cenchrus clandestinus?Hochst. ex Chiov.) of intermediate sward heights. The treatments consisted of five pre-grazing sward heights (10, 15, 20, 25, and 30 cm) randomly assigned to two of ten paddocks. The experimental design included two measurements of each paddock at different periods and times of day. Three Holstein heifers (440 ± 42 kg body weight) were used to determine the STIR, which was estimated using the double-weighing technique with correction for insensible weight losses. The bite mass (BM), bite rate (BR), sward structural characteristics, and nutritional value of herbage samples were assessed. The data were analyzed using mixed models with a factorial arrangement of five sward heights, two times of day, and two evaluation periods. The sward height of Kikuyu grass that maximized both STIRs was approximately 20 cm. The STIR of the DM was 30% and 15% lower than the maximum in the shortest and tallest swards tested, respectively. In swards shorter than 20 cm, the STIR was lower because the BM decreased with sward height, whereas in those greater than 20 cm, the lower BM and STIR of DM was explained by a decrease in bulk density and bite volume. The top stratum was composed mainly of highly digestible leaf blades with similar nutrient content across sward heights; therefore the STIR of digestible OM was also maximized at 20 cm. Hence, the optimal pre-grazing sward height of Kikuyu grass should be managed at 20 cm under rotational stocking systems to maximize nutrient intake rate of dairy heifers. MenosUnderstanding the grazing process and animal response to sward structures (e.g., sward height) is key to setting targets for efficient grazing management. We hypothesized that the short-term intake rate (STIR) of dry matter (DM) and digestible organic matter (OM) by dairy heifers is maximized with Kikuyu grass (Cenchrus clandestinus?Hochst. ex Chiov.) of intermediate sward heights. The treatments consisted of five pre-grazing sward heights (10, 15, 20, 25, and 30 cm) randomly assigned to two of ten paddocks. The experimental design included two measurements of each paddock at different periods and times of day. Three Holstein heifers (440 ± 42 kg body weight) were used to determine the STIR, which was estimated using the double-weighing technique with correction for insensible weight losses. The bite mass (BM), bite rate (BR), sward structural characteristics, and nutritional value of herbage samples were assessed. The data were analyzed using mixed models with a factorial arrangement of five sward heights, two times of day, and two evaluation periods. The sward height of Kikuyu grass that maximized both STIRs was approximately 20 cm. The STIR of the DM was 30% and 15% lower than the maximum in the shortest and tallest swards tested, respectively. In swards shorter than 20 cm, the STIR was lower because the BM decreased with sward height, whereas in those greater than 20 cm, the lower BM and STIR of DM was explained by a decrease in bulk density and bite volume. The top stra... Mostrar Tudo |
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Thesagro: |
Gado Leiteiro; Pastagem. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02663naa a2200253 a 4500
001 2149257
005 2022-12-06
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi. org/10.1371/journal.pone.0269716$2DOI
100 1 $aMARÍN GÓMEZ, A.
245 $aDetermining the pre-grazing sward height of Kikuyu grass (Cenchrus clandestinus - Hochst. ex Chiov.) for optimizing nutrient intake rate of dairy heifers.$h[electronic resource]
260 $c2022
520 $aUnderstanding the grazing process and animal response to sward structures (e.g., sward height) is key to setting targets for efficient grazing management. We hypothesized that the short-term intake rate (STIR) of dry matter (DM) and digestible organic matter (OM) by dairy heifers is maximized with Kikuyu grass (Cenchrus clandestinus?Hochst. ex Chiov.) of intermediate sward heights. The treatments consisted of five pre-grazing sward heights (10, 15, 20, 25, and 30 cm) randomly assigned to two of ten paddocks. The experimental design included two measurements of each paddock at different periods and times of day. Three Holstein heifers (440 ± 42 kg body weight) were used to determine the STIR, which was estimated using the double-weighing technique with correction for insensible weight losses. The bite mass (BM), bite rate (BR), sward structural characteristics, and nutritional value of herbage samples were assessed. The data were analyzed using mixed models with a factorial arrangement of five sward heights, two times of day, and two evaluation periods. The sward height of Kikuyu grass that maximized both STIRs was approximately 20 cm. The STIR of the DM was 30% and 15% lower than the maximum in the shortest and tallest swards tested, respectively. In swards shorter than 20 cm, the STIR was lower because the BM decreased with sward height, whereas in those greater than 20 cm, the lower BM and STIR of DM was explained by a decrease in bulk density and bite volume. The top stratum was composed mainly of highly digestible leaf blades with similar nutrient content across sward heights; therefore the STIR of digestible OM was also maximized at 20 cm. Hence, the optimal pre-grazing sward height of Kikuyu grass should be managed at 20 cm under rotational stocking systems to maximize nutrient intake rate of dairy heifers.
650 $aGado Leiteiro
650 $aPastagem
700 1 $aLACA, E. A.
700 1 $aBALDISSERA, T. C.
700 1 $aPINTO, C. E.
700 1 $aGARAGORRY, F. C.
700 1 $aZUBIETA, A. S.
700 1 $aBREMM, C.
700 1 $aBINDELLE, J.
700 1 $aCARVALHO, P. C. de F.
773 $tPLos One$gv. 17, n. 7, e0269716, July 2022.
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